Construct: shRNA TRCN0000109162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009224.3-446s1c1
DNA Barcode:
CGATGCCTTCAAGACTCTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snrnp70 (20637)
Gene Description:
small nuclear ribonucleoprotein 70 (U1)
Transcript:
RefSeq NM_009224.3 (NON-CURRENT)
Match location:
Position 446 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGCCTTCAAGACTCTGTT
Hairpin Sequence:
5'-CCGG-CGATGCCTTCAAGACTCTGTT-CTCGAG-AACAGAGTCTTGAAGGCATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGCCTTCAAGACTCTGTTCTCGAGAACAGAGTCTTGAAGGCATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGCCTTCAAGACTCTGTTCTCGAGAACAGAGTCTTGAAGGCATCG-3'

Other clones with same target sequence:

  1. TRCN0000287138