Construct: shRNA TRCN0000109274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008956.1-1123s1c1
DNA Barcode:
GCGGGTGAAGATCCTGTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptbp1 (19205)
Gene Description:
polypyrimidine tract binding protein 1
Transcript:
RefSeq NM_008956.1 (NON-CURRENT)
Match location:
Position 1123 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGGTGAAGATCCTGTTCAA
Hairpin Sequence:
5'-CCGG-GCGGGTGAAGATCCTGTTCAA-CTCGAG-TTGAACAGGATCTTCACCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGGTGAAGATCCTGTTCAACTCGAGTTGAACAGGATCTTCACCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGGTGAAGATCCTGTTCAACTCGAGTTGAACAGGATCTTCACCCGC-3'

Other clones with same target sequence:

(none)