Construct: shRNA TRCN0000109291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010312.3-989s1c1
DNA Barcode:
GCTCATGTATTCCCACGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnb2 (14693)
Gene Description:
guanine nucleotide binding protein (G protein), beta 2
Transcript:
RefSeq NM_010312.3 (NON-CURRENT)
Match location:
Position 989 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATGTATTCCCACGACAA
Hairpin Sequence:
5'-CCGG-GCTCATGTATTCCCACGACAA-CTCGAG-TTGTCGTGGGAATACATGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATGTATTCCCACGACAACTCGAGTTGTCGTGGGAATACATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATGTATTCCCACGACAACTCGAGTTGTCGTGGGAATACATGAGC-3'

Other clones with same target sequence:

  1. TRCN0000309853