Construct: shRNA TRCN0000109313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010855.2-334s1c1
DNA Barcode:
CCTCCCAAGTACGACAAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myh4 (17884)
Gene Description:
myosin, heavy polypeptide 4, skeletal muscle
Transcript:
RefSeq NM_010855.2 (NON-CURRENT)
Match location:
Position 334 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCAAGTACGACAAGATT
Hairpin Sequence:
5'-CCGG-CCTCCCAAGTACGACAAGATT-CTCGAG-AATCTTGTCGTACTTGGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCAAGTACGACAAGATTCTCGAGAATCTTGTCGTACTTGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCAAGTACGACAAGATTCTCGAGAATCTTGTCGTACTTGGGAGG-3'

Other clones with same target sequence:

(none)