Construct: shRNA TRCN0000109317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029337.1-6165s1c1
DNA Barcode:
GCTGACTTTATGGAACAGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ep400 (75560)
Gene Description:
E1A binding protein p400
Transcript:
RefSeq NM_029337.1 (NON-CURRENT)
Match location:
Position 6165 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACTTTATGGAACAGCTT
Hairpin Sequence:
5'-CCGG-GCTGACTTTATGGAACAGCTT-CTCGAG-AAGCTGTTCCATAAAGTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACTTTATGGAACAGCTTCTCGAGAAGCTGTTCCATAAAGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACTTTATGGAACAGCTTCTCGAGAAGCTGTTCCATAAAGTCAGC-3'

Other clones with same target sequence:

(none)