Construct: shRNA TRCN0000109331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013531.2-260s1c1
DNA Barcode:
GCCGAATACAAATGCGAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnb4 (14696)
Gene Description:
guanine nucleotide binding protein (G protein), beta 4
Transcript:
RefSeq NM_013531.2 (NON-CURRENT)
Match location:
Position 260 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAATACAAATGCGAACAA
Hairpin Sequence:
5'-CCGG-GCCGAATACAAATGCGAACAA-CTCGAG-TTGTTCGCATTTGTATTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAATACAAATGCGAACAACTCGAGTTGTTCGCATTTGTATTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAATACAAATGCGAACAACTCGAGTTGTTCGCATTTGTATTCGGC-3'

Other clones with same target sequence:

  1. TRCN0000334625