Construct: shRNA TRCN0000109340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030732.2-2319s1c1
DNA Barcode:
GCATGAAGTAAGGGAGTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbl1xr1 (81004)
Gene Description:
transducin (beta)-like 1X-linked receptor 1
Transcript:
RefSeq NM_030732.2 (NON-CURRENT)
Match location:
Position 2319 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGAAGTAAGGGAGTGAAT
Hairpin Sequence:
5'-CCGG-GCATGAAGTAAGGGAGTGAAT-CTCGAG-ATTCACTCCCTTACTTCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGAAGTAAGGGAGTGAATCTCGAGATTCACTCCCTTACTTCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGAAGTAAGGGAGTGAATCTCGAGATTCACTCCCTTACTTCATGC-3'

Other clones with same target sequence:

  1. TRCN0000317949