Construct: shRNA TRCN0000109354

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010313.1-841s1c1
DNA Barcode:
GAATCGGATGTCAACAGTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnb5 (14697)
Gene Description:
guanine nucleotide binding protein (G protein), beta 5
Transcript:
RefSeq NM_010313.1 (NON-CURRENT)
Match location:
Position 841 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109354)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATCGGATGTCAACAGTGTT
Hairpin Sequence:
5'-CCGG-GAATCGGATGTCAACAGTGTT-CTCGAG-AACACTGTTGACATCCGATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATCGGATGTCAACAGTGTTCTCGAGAACACTGTTGACATCCGATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATCGGATGTCAACAGTGTTCTCGAGAACACTGTTGACATCCGATTC-3'

Other clones with same target sequence:

  1. TRCN0000332246