Construct: shRNA TRCN0000109412

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007615.1-2545s1c1
DNA Barcode:
GCTCGGAACAAAGAGTTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctnnd1 (12388)
Gene Description:
catenin (cadherin associated protein), delta 1
Transcript:
RefSeq NM_007615.1 (NON-CURRENT)
Match location:
Position 2545 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109412)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGGAACAAAGAGTTAATT
Hairpin Sequence:
5'-CCGG-GCTCGGAACAAAGAGTTAATT-CTCGAG-AATTAACTCTTTGTTCCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGGAACAAAGAGTTAATTCTCGAGAATTAACTCTTTGTTCCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGGAACAAAGAGTTAATTCTCGAGAATTAACTCTTTGTTCCGAGC-3'

Other clones with same target sequence:

  1. TRCN0000332237