Construct: shRNA TRCN0000109427

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007962.2-393s1c1
DNA Barcode:
GCTAACTGTGACGTGGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mpzl2 (14012)
Gene Description:
myelin protein zero-like 2
Transcript:
RefSeq NM_007962.2 (NON-CURRENT)
Match location:
Position 393 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109427)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAACTGTGACGTGGAATTT
Hairpin Sequence:
5'-CCGG-GCTAACTGTGACGTGGAATTT-CTCGAG-AAATTCCACGTCACAGTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAACTGTGACGTGGAATTTCTCGAGAAATTCCACGTCACAGTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAACTGTGACGTGGAATTTCTCGAGAAATTCCACGTCACAGTTAGC-3'

Other clones with same target sequence:

(none)