Construct: shRNA TRCN0000109431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005868.1-1130s1c1
DNA Barcode:
GCCGGAAACTATTGGTTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Erbin (59079)
Gene Description:
Erbb2 interacting protein
Transcript:
RefSeq NM_001005868.1 (NON-CURRENT)
Match location:
Position 1130 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGGAAACTATTGGTTCATT
Hairpin Sequence:
5'-CCGG-GCCGGAAACTATTGGTTCATT-CTCGAG-AATGAACCAATAGTTTCCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGGAAACTATTGGTTCATTCTCGAGAATGAACCAATAGTTTCCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGGAAACTATTGGTTCATTCTCGAGAATGAACCAATAGTTTCCGGC-3'

Other clones with same target sequence:

  1. TRCN0000317493