Construct: shRNA TRCN0000109458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016697.2-1178s1c1
DNA Barcode:
GCCAATATAGATCTGCTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpc3 (14734)
Gene Description:
glypican 3
Transcript:
RefSeq NM_016697.2 (NON-CURRENT)
Match location:
Position 1178 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATATAGATCTGCTTATT
Hairpin Sequence:
5'-CCGG-GCCAATATAGATCTGCTTATT-CTCGAG-AATAAGCAGATCTATATTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATATAGATCTGCTTATTCTCGAGAATAAGCAGATCTATATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATATAGATCTGCTTATTCTCGAGAATAAGCAGATCTATATTGGC-3'

Other clones with same target sequence:

(none)