Construct: shRNA TRCN0000109510

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138673.1-7844s1c1
DNA Barcode:
GTATGATAACTCAGAAGCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stab2 (192188)
Gene Description:
stabilin 2
Transcript:
RefSeq NM_138673.1 (NON-CURRENT)
Match location:
Position 7844 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109510)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATGATAACTCAGAAGCCAT
Hairpin Sequence:
5'-CCGG-GTATGATAACTCAGAAGCCAT-CTCGAG-ATGGCTTCTGAGTTATCATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATGATAACTCAGAAGCCATCTCGAGATGGCTTCTGAGTTATCATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATGATAACTCAGAAGCCATCTCGAGATGGCTTCTGAGTTATCATAC-3'

Other clones with same target sequence:

(none)