Construct: shRNA TRCN0000109538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009929.2-1413s1c1
DNA Barcode:
GCGGAGACATAGAGAGCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col18a1 (12822)
Gene Description:
collagen, type XVIII, alpha 1
Transcript:
RefSeq NM_009929.2 (NON-CURRENT)
Match location:
Position 1413 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAGACATAGAGAGCCTTA
Hairpin Sequence:
5'-CCGG-GCGGAGACATAGAGAGCCTTA-CTCGAG-TAAGGCTCTCTATGTCTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAGACATAGAGAGCCTTACTCGAGTAAGGCTCTCTATGTCTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAGACATAGAGAGCCTTACTCGAGTAAGGCTCTCTATGTCTCCGC-3'

Other clones with same target sequence:

  1. TRCN0000326585