Construct: shRNA TRCN0000109557

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175522.2-2189s1c1
DNA Barcode:
CCCTGTATACAAAGATGCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elfn1 (243312)
Gene Description:
leucine rich repeat and fibronectin type III, extracellular 1
Transcript:
RefSeq NM_175522.2 (NON-CURRENT)
Match location:
Position 2189 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109557)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTATACAAAGATGCCTT
Hairpin Sequence:
5'-CCGG-CCCTGTATACAAAGATGCCTT-CTCGAG-AAGGCATCTTTGTATACAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTATACAAAGATGCCTTCTCGAGAAGGCATCTTTGTATACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTATACAAAGATGCCTTCTCGAGAAGGCATCTTTGTATACAGGG-3'

Other clones with same target sequence:

(none)