Construct: shRNA TRCN0000109560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183141.2-3397s1c1
DNA Barcode:
CCTCTTGTTCAGAGAGGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elfn2 (207393)
Gene Description:
leucine rich repeat and fibronectin type III, extracellular 2
Transcript:
RefSeq NM_183141.2 (CURRENT)
Match location:
Position 3397 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTGTTCAGAGAGGCTAT
Hairpin Sequence:
5'-CCGG-CCTCTTGTTCAGAGAGGCTAT-CTCGAG-ATAGCCTCTCTGAACAAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTGTTCAGAGAGGCTATCTCGAGATAGCCTCTCTGAACAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTGTTCAGAGAGGCTATCTCGAGATAGCCTCTCTGAACAAGAGG-3'

Other clones with same target sequence:

(none)