Construct: shRNA TRCN0000109622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033474.2-2876s1c1
DNA Barcode:
CTGGTAGACAAGAGCCTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arvcf (11877)
Gene Description:
armadillo repeat gene deleted in velocardiofacial syndrome
Transcript:
RefSeq NM_033474.2 (NON-CURRENT)
Match location:
Position 2876 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGTAGACAAGAGCCTTGAT
Hairpin Sequence:
5'-CCGG-CTGGTAGACAAGAGCCTTGAT-CTCGAG-ATCAAGGCTCTTGTCTACCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGTAGACAAGAGCCTTGATCTCGAGATCAAGGCTCTTGTCTACCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGTAGACAAGAGCCTTGATCTCGAGATCAAGGCTCTTGTCTACCAG-3'

Other clones with same target sequence:

(none)