Construct: shRNA TRCN0000109675

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177649.3-2719s1c1
DNA Barcode:
CCCTTTGATGGCAAAGCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
BC034076 (227712)
Gene Description:
cDNA sequence BC034076
Transcript:
RefSeq NM_177649.3 (NON-CURRENT)
Match location:
Position 2719 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109675)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTTGATGGCAAAGCCAAT
Hairpin Sequence:
5'-CCGG-CCCTTTGATGGCAAAGCCAAT-CTCGAG-ATTGGCTTTGCCATCAAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTTGATGGCAAAGCCAATCTCGAGATTGGCTTTGCCATCAAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTTGATGGCAAAGCCAATCTCGAGATTGGCTTTGCCATCAAAGGG-3'

Other clones with same target sequence:

(none)