Construct: shRNA TRCN0000109726

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010730.1-982s1c1
DNA Barcode:
CCGTTCGGAAATTGACATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Anxa1 (16952)
Gene Description:
annexin A1
Transcript:
RefSeq NM_010730.1 (NON-CURRENT)
Match location:
Position 982 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109726)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTCGGAAATTGACATGAA
Hairpin Sequence:
5'-CCGG-CCGTTCGGAAATTGACATGAA-CTCGAG-TTCATGTCAATTTCCGAACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTCGGAAATTGACATGAACTCGAGTTCATGTCAATTTCCGAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTCGGAAATTGACATGAACTCGAGTTCATGTCAATTTCCGAACGG-3'

Other clones with same target sequence:

  1. TRCN0000327421