Construct: shRNA TRCN0000109729

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010730.1-656s1c1
DNA Barcode:
GTGAATCAAGATTTGGCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Anxa1 (16952)
Gene Description:
annexin A1
Transcript:
RefSeq NM_010730.1 (NON-CURRENT)
Match location:
Position 656 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109729)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAATCAAGATTTGGCTGAT
Hairpin Sequence:
5'-CCGG-GTGAATCAAGATTTGGCTGAT-CTCGAG-ATCAGCCAAATCTTGATTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAATCAAGATTTGGCTGATCTCGAGATCAGCCAAATCTTGATTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAATCAAGATTTGGCTGATCTCGAGATCAGCCAAATCTTGATTCAC-3'

Other clones with same target sequence:

  1. TRCN0000327418