Construct: shRNA TRCN0000109845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153197.2-170s1c1
DNA Barcode:
CCTCTTTACCTCACTCATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clec4a3 (73149)
Gene Description:
C-type lectin domain family 4, member a3
Transcript:
RefSeq NM_153197.2 (NON-CURRENT)
Match location:
Position 170 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTTACCTCACTCATAAT
Hairpin Sequence:
5'-CCGG-CCTCTTTACCTCACTCATAAT-CTCGAG-ATTATGAGTGAGGTAAAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTTACCTCACTCATAATCTCGAGATTATGAGTGAGGTAAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTTACCTCACTCATAATCTCGAGATTATGAGTGAGGTAAAGAGG-3'

Other clones with same target sequence:

(none)