Construct: shRNA TRCN0000109858

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177593.1-375s1c1
DNA Barcode:
CCTAAGGACACTCGTTGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm4763 (210155)
Gene Description:
predicted gene 4763
Transcript:
RefSeq NM_177593.1 (CURRENT)
Match location:
Position 375 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109858)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAGGACACTCGTTGCTAT
Hairpin Sequence:
5'-CCGG-CCTAAGGACACTCGTTGCTAT-CTCGAG-ATAGCAACGAGTGTCCTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAGGACACTCGTTGCTATCTCGAGATAGCAACGAGTGTCCTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAGGACACTCGTTGCTATCTCGAGATAGCAACGAGTGTCCTTAGG-3'

Other clones with same target sequence:

(none)