Construct: shRNA TRCN0000109883

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026862.3-1880s1c1
DNA Barcode:
GCACCTGATGAGATTTGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cd177 (68891)
Gene Description:
CD177 antigen
Transcript:
RefSeq NM_026862.3 (CURRENT)
Match location:
Position 1880 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109883)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTGATGAGATTTGTCAA
Hairpin Sequence:
5'-CCGG-GCACCTGATGAGATTTGTCAA-CTCGAG-TTGACAAATCTCATCAGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTGATGAGATTTGTCAACTCGAGTTGACAAATCTCATCAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTGATGAGATTTGTCAACTCGAGTTGACAAATCTCATCAGGTGC-3'

Other clones with same target sequence:

(none)