Construct: shRNA TRCN0000109946

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029741.1-1229s1c1
DNA Barcode:
GCTAACATGAAGAAACTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppfia3 (76787)
Gene Description:
protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
Transcript:
RefSeq NM_029741.1 (NON-CURRENT)
Match location:
Position 1229 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109946)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAACATGAAGAAACTTCAA
Hairpin Sequence:
5'-CCGG-GCTAACATGAAGAAACTTCAA-CTCGAG-TTGAAGTTTCTTCATGTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAACATGAAGAAACTTCAACTCGAGTTGAAGTTTCTTCATGTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAACATGAAGAAACTTCAACTCGAGTTGAAGTTTCTTCATGTTAGC-3'

Other clones with same target sequence:

(none)