Construct: shRNA TRCN0000109956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139232.1-1110s1c1
DNA Barcode:
CCTTCCATAGTAAATTCCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fgd4 (224014)
Gene Description:
FYVE, RhoGEF and PH domain containing 4
Transcript:
RefSeq NM_139232.1 (NON-CURRENT)
Match location:
Position 1110 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCATAGTAAATTCCTAT
Hairpin Sequence:
5'-CCGG-CCTTCCATAGTAAATTCCTAT-CTCGAG-ATAGGAATTTACTATGGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCATAGTAAATTCCTATCTCGAGATAGGAATTTACTATGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCATAGTAAATTCCTATCTCGAGATAGGAATTTACTATGGAAGG-3'

Other clones with same target sequence:

(none)