Construct: shRNA TRCN0000109974

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024477.2-3479s1c1
DNA Barcode:
CAAGAGGAAGTGATCCTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc28 (209683)
Gene Description:
tetratricopeptide repeat domain 28
Transcript:
RefSeq NM_024477.2 (NON-CURRENT)
Match location:
Position 3479 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109974)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAGGAAGTGATCCTGAAA
Hairpin Sequence:
5'-CCGG-CAAGAGGAAGTGATCCTGAAA-CTCGAG-TTTCAGGATCACTTCCTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAGGAAGTGATCCTGAAACTCGAGTTTCAGGATCACTTCCTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAGGAAGTGATCCTGAAACTCGAGTTTCAGGATCACTTCCTCTTG-3'

Other clones with same target sequence:

(none)