Construct: shRNA TRCN0000109997

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175930.3-1956s1c1
DNA Barcode:
GCCGTCACACTGTTGATGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rapgef5 (217944)
Gene Description:
Rap guanine nucleotide exchange factor (GEF) 5
Transcript:
RefSeq NM_175930.3 (NON-CURRENT)
Match location:
Position 1956 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000109997)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTCACACTGTTGATGAAT
Hairpin Sequence:
5'-CCGG-GCCGTCACACTGTTGATGAAT-CTCGAG-ATTCATCAACAGTGTGACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTCACACTGTTGATGAATCTCGAGATTCATCAACAGTGTGACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTCACACTGTTGATGAATCTCGAGATTCATCAACAGTGTGACGGC-3'

Other clones with same target sequence:

(none)