Construct: shRNA TRCN0000110024

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008001.2-2243s1c1
DNA Barcode:
GCAAAGAATGGGACCACTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fgd1 (14163)
Gene Description:
FYVE, RhoGEF and PH domain containing 1
Transcript:
RefSeq NM_008001.2 (NON-CURRENT)
Match location:
Position 2243 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110024)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGAATGGGACCACTCAA
Hairpin Sequence:
5'-CCGG-GCAAAGAATGGGACCACTCAA-CTCGAG-TTGAGTGGTCCCATTCTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGAATGGGACCACTCAACTCGAGTTGAGTGGTCCCATTCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGAATGGGACCACTCAACTCGAGTTGAGTGGTCCCATTCTTTGC-3'

Other clones with same target sequence:

(none)