Construct: shRNA TRCN0000110037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177611.3-2071s1c1
DNA Barcode:
CCTGTGCAATCATGCTCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psd4 (215632)
Gene Description:
pleckstrin and Sec7 domain containing 4
Transcript:
RefSeq NM_177611.3 (CURRENT)
Match location:
Position 2071 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGCAATCATGCTCCTTA
Hairpin Sequence:
5'-CCGG-CCTGTGCAATCATGCTCCTTA-CTCGAG-TAAGGAGCATGATTGCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGCAATCATGCTCCTTACTCGAGTAAGGAGCATGATTGCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGCAATCATGCTCCTTACTCGAGTAAGGAGCATGATTGCACAGG-3'

Other clones with same target sequence:

(none)