Construct: shRNA TRCN0000110062

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012026.1-2001s1c1
DNA Barcode:
CGGAACAAATCTAAGATGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgef28 (110596)
Gene Description:
Rho guanine nucleotide exchange factor (GEF) 28
Transcript:
RefSeq NM_012026.1 (NON-CURRENT)
Match location:
Position 2001 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110062)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAACAAATCTAAGATGAAA
Hairpin Sequence:
5'-CCGG-CGGAACAAATCTAAGATGAAA-CTCGAG-TTTCATCTTAGATTTGTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAACAAATCTAAGATGAAACTCGAGTTTCATCTTAGATTTGTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAACAAATCTAAGATGAAACTCGAGTTTCATCTTAGATTTGTTCCG-3'

Other clones with same target sequence:

(none)