Construct: shRNA TRCN0000110077

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177698.2-702s1c1
DNA Barcode:
GCCGAACGTGTTTAAACTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psd3 (234353)
Gene Description:
pleckstrin and Sec7 domain containing 3
Transcript:
RefSeq NM_177698.2 (NON-CURRENT)
Match location:
Position 702 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110077)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAACGTGTTTAAACTCAA
Hairpin Sequence:
5'-CCGG-GCCGAACGTGTTTAAACTCAA-CTCGAG-TTGAGTTTAAACACGTTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAACGTGTTTAAACTCAACTCGAGTTGAGTTTAAACACGTTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAACGTGTTTAAACTCAACTCGAGTTGAGTTTAAACACGTTCGGC-3'

Other clones with same target sequence:

  1. TRCN0000298437