Construct: shRNA TRCN0000110085

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023622.2-2237s1c1
DNA Barcode:
CTTGGATTCTACTACTATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rgl3 (71746)
Gene Description:
ral guanine nucleotide dissociation stimulator-like 3
Transcript:
RefSeq NM_023622.2 (NON-CURRENT)
Match location:
Position 2237 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110085)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGGATTCTACTACTATCAA
Hairpin Sequence:
5'-CCGG-CTTGGATTCTACTACTATCAA-CTCGAG-TTGATAGTAGTAGAATCCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGGATTCTACTACTATCAACTCGAGTTGATAGTAGTAGAATCCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGGATTCTACTACTATCAACTCGAGTTGATAGTAGTAGAATCCAAG-3'

Other clones with same target sequence:

(none)