Construct: shRNA TRCN0000110140
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_177657.2-3738s1c1
- DNA Barcode:
- TGACAGGATTGACAGAGGGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- D630003M21Rik (228846)
- Gene Description:
- RIKEN cDNA D630003M21 gene
- Transcript:
- RefSeq NM_177657.2 (NON-CURRENT)
- Match location:
- Position 3738 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000110140)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGACAGGATTGACAGAGGGAT
- Hairpin Sequence:
-
5'-CCGG-TGACAGGATTGACAGAGGGAT-CTCGAG-ATCCCTCTGTCAATCCTGTCA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGACAGGATTGACAGAGGGATCTCGAGATCCCTCTGTCAATCCTGTCATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGACAGGATTGACAGAGGGATCTCGAGATCCCTCTGTCAATCCTGTCA-3'
Other clones with same target sequence:
(none)
