Construct: shRNA TRCN0000110153

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133962.3-3192s1c1
DNA Barcode:
CCACCACAGATGAAGTTAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgef18 (102098)
Gene Description:
rho/rac guanine nucleotide exchange factor (GEF) 18
Transcript:
RefSeq NM_133962.3 (CURRENT)
Match location:
Position 3192 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110153)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCACAGATGAAGTTAGTA
Hairpin Sequence:
5'-CCGG-CCACCACAGATGAAGTTAGTA-CTCGAG-TACTAACTTCATCTGTGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCACAGATGAAGTTAGTACTCGAGTACTAACTTCATCTGTGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCACAGATGAAGTTAGTACTCGAGTACTAACTTCATCTGTGGTGG-3'

Other clones with same target sequence:

(none)