Construct: shRNA TRCN0000110183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003912.1-1091s1c1
DNA Barcode:
CCAGCTTACTTGGTAGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgef11 (213498)
Gene Description:
Rho guanine nucleotide exchange factor (GEF) 11
Transcript:
RefSeq NM_001003912.1 (CURRENT)
Match location:
Position 1091 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTTACTTGGTAGTATTT
Hairpin Sequence:
5'-CCGG-CCAGCTTACTTGGTAGTATTT-CTCGAG-AAATACTACCAAGTAAGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTTACTTGGTAGTATTTCTCGAGAAATACTACCAAGTAAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTTACTTGGTAGTATTTCTCGAGAAATACTACCAAGTAAGCTGG-3'

Other clones with same target sequence:

(none)