Construct: shRNA TRCN0000110244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001009819.1-957s1c1
DNA Barcode:
CGACGAAAGCCACCTTAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
A3galt2 (215493)
Gene Description:
alpha 1,3-galactosyltransferase 2 (isoglobotriaosylceramide synthase)
Transcript:
RefSeq NM_001009819.1 (NON-CURRENT)
Match location:
Position 957 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGAAAGCCACCTTAACAA
Hairpin Sequence:
5'-CCGG-CGACGAAAGCCACCTTAACAA-CTCGAG-TTGTTAAGGTGGCTTTCGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGAAAGCCACCTTAACAACTCGAGTTGTTAAGGTGGCTTTCGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGAAAGCCACCTTAACAACTCGAGTTGTTAAGGTGGCTTTCGTCG-3'

Other clones with same target sequence:

(none)