Construct: shRNA TRCN0000110265
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_139272.2-3025s1c1
- DNA Barcode:
- GCAGGAATTATGGGTAGAGTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Galnt2 (108148)
- Gene Description:
- UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2
- Transcript:
- RefSeq NM_139272.2 (CURRENT)
- Match location:
- Position 3025 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000110265)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGGAATTATGGGTAGAGTT
- Hairpin Sequence:
-
5'-CCGG-GCAGGAATTATGGGTAGAGTT-CTCGAG-AACTCTACCCATAATTCCTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGGAATTATGGGTAGAGTTCTCGAGAACTCTACCCATAATTCCTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGGAATTATGGGTAGAGTTCTCGAGAACTCTACCCATAATTCCTGC-3'
Other clones with same target sequence:
-
TRCN0000318297
