Construct: shRNA TRCN0000110272
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_012028.2-994s1c1
- DNA Barcode:
- CCTATCATTACTATGAGCCTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- St6galnac5 (26938)
- Gene Description:
- ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5
- Transcript:
- RefSeq NM_012028.2 (NON-CURRENT)
- Match location:
- Position 994 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000110272)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTATCATTACTATGAGCCTT
- Hairpin Sequence:
-
5'-CCGG-CCTATCATTACTATGAGCCTT-CTCGAG-AAGGCTCATAGTAATGATAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTATCATTACTATGAGCCTTCTCGAGAAGGCTCATAGTAATGATAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTATCATTACTATGAGCCTTCTCGAGAAGGCTCATAGTAATGATAGG-3'
Other clones with same target sequence:
(none)
