Construct: shRNA TRCN0000110281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172693.2-1481s1c1
DNA Barcode:
CCCGGAAAGAAATACGCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galnt12 (230145)
Gene Description:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12
Transcript:
RefSeq NM_172693.2 (NON-CURRENT)
Match location:
Position 1481 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGAAAGAAATACGCTATA
Hairpin Sequence:
5'-CCGG-CCCGGAAAGAAATACGCTATA-CTCGAG-TATAGCGTATTTCTTTCCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGAAAGAAATACGCTATACTCGAGTATAGCGTATTTCTTTCCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGAAAGAAATACGCTATACTCGAGTATAGCGTATTTCTTTCCGGG-3'

Other clones with same target sequence:

(none)