Construct: shRNA TRCN0000110283

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172693.2-1183s1c1
DNA Barcode:
GAACTCTACTACCACCGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galnt12 (230145)
Gene Description:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12
Transcript:
RefSeq NM_172693.2 (NON-CURRENT)
Match location:
Position 1183 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110283)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTCTACTACCACCGCAAT
Hairpin Sequence:
5'-CCGG-GAACTCTACTACCACCGCAAT-CTCGAG-ATTGCGGTGGTAGTAGAGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTCTACTACCACCGCAATCTCGAGATTGCGGTGGTAGTAGAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTCTACTACCACCGCAATCTCGAGATTGCGGTGGTAGTAGAGTTC-3'

Other clones with same target sequence:

(none)