Construct: shRNA TRCN0000110291
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_013814.2-1454s1c1
- DNA Barcode:
- GCCTAAGACGTAAACTACAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Galnt1 (14423)
- Gene Description:
- UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1
- Transcript:
- RefSeq NM_013814.2 (NON-CURRENT)
- Match location:
- Position 1454 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000110291)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTAAGACGTAAACTACAAT
- Hairpin Sequence:
-
5'-CCGG-GCCTAAGACGTAAACTACAAT-CTCGAG-ATTGTAGTTTACGTCTTAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTAAGACGTAAACTACAATCTCGAGATTGTAGTTTACGTCTTAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTAAGACGTAAACTACAATCTCGAGATTGTAGTTTACGTCTTAGGC-3'
Other clones with same target sequence:
-
TRCN0000335167
