Construct: shRNA TRCN0000110293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013814.2-1779s1c1
DNA Barcode:
CCCAGTGAAATTAACCCTGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galnt1 (14423)
Gene Description:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1
Transcript:
RefSeq NM_013814.2 (NON-CURRENT)
Match location:
Position 1779 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTGAAATTAACCCTGCA
Hairpin Sequence:
5'-CCGG-CCCAGTGAAATTAACCCTGCA-CTCGAG-TGCAGGGTTAATTTCACTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTGAAATTAACCCTGCACTCGAGTGCAGGGTTAATTTCACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTGAAATTAACCCTGCACTCGAGTGCAGGGTTAATTTCACTGGG-3'

Other clones with same target sequence:

  1. TRCN0000335092