Construct: shRNA TRCN0000110303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025442.1-233s1c1
DNA Barcode:
CCTACGAAACAGCTATCTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alg5 (66248)
Gene Description:
asparagine-linked glycosylation 5 (dolichyl-phosphate beta-glucosyltransferase)
Transcript:
RefSeq NM_025442.1 (NON-CURRENT)
Match location:
Position 233 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACGAAACAGCTATCTGTT
Hairpin Sequence:
5'-CCGG-CCTACGAAACAGCTATCTGTT-CTCGAG-AACAGATAGCTGTTTCGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACGAAACAGCTATCTGTTCTCGAGAACAGATAGCTGTTTCGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACGAAACAGCTATCTGTTCTCGAGAACAGATAGCTGTTTCGTAGG-3'

Other clones with same target sequence:

  1. TRCN0000302764