Construct: shRNA TRCN0000110308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011374.2-1495s1c1
DNA Barcode:
CGCTTGTCTACAGGACTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
St8sia1 (20449)
Gene Description:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
Transcript:
RefSeq NM_011374.2 (CURRENT)
Match location:
Position 1495 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTTGTCTACAGGACTCTTT
Hairpin Sequence:
5'-CCGG-CGCTTGTCTACAGGACTCTTT-CTCGAG-AAAGAGTCCTGTAGACAAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTTGTCTACAGGACTCTTTCTCGAGAAAGAGTCCTGTAGACAAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTTGTCTACAGGACTCTTTCTCGAGAAAGAGTCCTGTAGACAAGCG-3'

Other clones with same target sequence:

(none)