Construct: shRNA TRCN0000110355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201642.1-1658s1c1
DNA Barcode:
GAAGGAGAAGTATTAGTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ugt1a7c (394432)
Gene Description:
UDP glucuronosyltransferase 1 family, polypeptide A7C
Transcript:
RefSeq NM_201642.1 (NON-CURRENT)
Match location:
Position 1658 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGAGAAGTATTAGTTCAT
Hairpin Sequence:
5'-CCGG-GAAGGAGAAGTATTAGTTCAT-CTCGAG-ATGAACTAATACTTCTCCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGAGAAGTATTAGTTCATCTCGAGATGAACTAATACTTCTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGAGAAGTATTAGTTCATCTCGAGATGAACTAATACTTCTCCTTC-3'

Other clones with same target sequence:

(none)