Construct: shRNA TRCN0000110377

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181728.1-921s1c1
DNA Barcode:
CCTGACAGAATGCCAGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Art3 (109979)
Gene Description:
ADP-ribosyltransferase 3
Transcript:
RefSeq NM_181728.1 (NON-CURRENT)
Match location:
Position 921 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110377)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGACAGAATGCCAGAAATT
Hairpin Sequence:
5'-CCGG-CCTGACAGAATGCCAGAAATT-CTCGAG-AATTTCTGGCATTCTGTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGACAGAATGCCAGAAATTCTCGAGAATTTCTGGCATTCTGTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGACAGAATGCCAGAAATTCTCGAGAATTTCTGGCATTCTGTCAGG-3'

Other clones with same target sequence:

(none)