Construct: shRNA TRCN0000110394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019934.1-75s1c1
DNA Barcode:
CTTCTACCTCTTCTTCGTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sec1 (56546)
Gene Description:
secretory blood group 1
Transcript:
RefSeq NM_019934.1 (NON-CURRENT)
Match location:
Position 75 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTACCTCTTCTTCGTGAT
Hairpin Sequence:
5'-CCGG-CTTCTACCTCTTCTTCGTGAT-CTCGAG-ATCACGAAGAAGAGGTAGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTACCTCTTCTTCGTGATCTCGAGATCACGAAGAAGAGGTAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTACCTCTTCTTCGTGATCTCGAGATCACGAAGAAGAGGTAGAAG-3'

Other clones with same target sequence:

(none)