Construct: shRNA TRCN0000110403

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021888.1-657s1c1
DNA Barcode:
CAAGGCACAGTTCTGGAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Qtrt1 (60507)
Gene Description:
queuine tRNA-ribosyltransferase catalytic subunit 1
Transcript:
RefSeq NM_021888.1 (NON-CURRENT)
Match location:
Position 657 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110403)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGGCACAGTTCTGGAAGAT
Hairpin Sequence:
5'-CCGG-CAAGGCACAGTTCTGGAAGAT-CTCGAG-ATCTTCCAGAACTGTGCCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGGCACAGTTCTGGAAGATCTCGAGATCTTCCAGAACTGTGCCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGGCACAGTTCTGGAAGATCTCGAGATCTTCCAGAACTGTGCCTTG-3'

Other clones with same target sequence:

(none)