Construct: shRNA TRCN0000110406

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153124.1-284s1c1
DNA Barcode:
GAATCGAACTTTGCTCTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
St8sia5 (225742)
Gene Description:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5
Transcript:
RefSeq NM_153124.1 (NON-CURRENT)
Match location:
Position 284 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000110406)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATCGAACTTTGCTCTTTAT
Hairpin Sequence:
5'-CCGG-GAATCGAACTTTGCTCTTTAT-CTCGAG-ATAAAGAGCAAAGTTCGATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATCGAACTTTGCTCTTTATCTCGAGATAAAGAGCAAAGTTCGATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATCGAACTTTGCTCTTTATCTCGAGATAAAGAGCAAAGTTCGATTC-3'

Other clones with same target sequence:

(none)