Construct: shRNA TRCN0000110409
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_153124.1-506s1c1
- DNA Barcode:
- CCAGATATGCAAATGGGCAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- St8sia5 (225742)
- Gene Description:
- ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5
- Transcript:
- RefSeq NM_153124.1 (NON-CURRENT)
- Match location:
- Position 506 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000110409)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGATATGCAAATGGGCAAT
- Hairpin Sequence:
-
5'-CCGG-CCAGATATGCAAATGGGCAAT-CTCGAG-ATTGCCCATTTGCATATCTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGATATGCAAATGGGCAATCTCGAGATTGCCCATTTGCATATCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGATATGCAAATGGGCAATCTCGAGATTGCCCATTTGCATATCTGG-3'
Other clones with same target sequence:
(none)
